The Analytical Genomics provides state-of-the-art genomic and sequencing services to support basic and translational medical research for academic institutions and industry sectors. We strive to enable access to cost-effective, customized genomic analyses, as a functional extension of your lab.

Instruments in self-service*

Genomics

The Analytical Genomics provides state-of-the-art genomic and sequencing services to support basic and translational medical research for academic institutions and industry sectors. We strive to enable access to cost-effective, customized genomic analyses, as a functional extension of your lab.

Contact

Carole Perrot, PhD
[email protected]
727-767-7023

NGS

  • 10X Genomics Chromium iX
  • Illumina NextSeq 550**
  • Illumina NextSeq 2000**

PCR

  • ThermoFisher QuantStudio 7 (qPCR)
  • BioRad C1000 Thermocycler

Nucleic acid and protein quality control

  • Agilent 4200 Tapestation
  • Invitrogen Qubit Flex Fluorometer
  • Nanodrop 8000 Spectrophotometer
  • Agilent Bioanalyzer
  • Covaris ME220

* All listed instruments available in self-service require training before use. 
** Sequencers are to be used under supervision only.


Core Services

NGS

Library Generation for Next-Generation Sequencing

Single Cell Sequencing using 10X Genomics Chromium iX

  • Single cell 3’ gene expression (compatible with ATAC-seq and CRISPR screening)
  • Single cell 5’ gene expression + V(D)J repertoire profiling
  • Single cell gene expression Flex (Fixed RNA profiling assay)

Other NGS Library generation

  • Transcriptome: total RNA, polyA-captured mRNA, small RNA

Next-Generation Sequencing on Illumina NextSeq550 and NextSeq2000

  • RNA-seq
  • Single cell sequencing
  • ChIP-seq
  • smRNA-seq
  • Whole Genome sequencing (WGS)
  • Whole Exome sequencing (WES)
  • Other sequencing upon request

Library quality control, quantification and pooling

  • Tapestation: library size, concentration, RNA integrity
  • Qubit Flex: Library concentration
  • qPCR: Library concentration
  • Library pooling prior to sequencing